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In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell ...
The epithelial–mesenchymal transition (EMT) is a process by which epithelial cells lose their cell polarity and cell–cell adhesion, and gain migratory and invasive properties to become mesenchymal stem cells; these are multipotent stromal cells that can differentiate into a variety of cell types.
In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. By causing certain cells or structures to take on contrasting colours, their form or position within a cell or tissue can be readily seen and studied. The usual purpose is to reveal cytological details that might otherwise not be ...
Supravital stain of a smear of human blood from a patient with hemolytic anemia. The reticulocytes are the cells with the dark blue dots and curved linear structures (reticulum) in the cytoplasm. Supravital staining is a method of staining used in microscopy to examine living cells that
In anatomy the term reticuloendothelial system (abbreviated RES), often associated nowadays with the mononuclear phagocyte system (MPS), was employed by the beginning of the 20th century to denote a system of specialised cells that effectively clear colloidal vital stains (so called because they stain living cells) from the blood circulation.
Freeze fracture enzyme cytochemistry was initially mentioned in the study of Pinto de silva in 1987. [3] It is a technique that allows the introduction of cytochemistry into a freeze fracture cell membrane. immunocytochemistry is used in this technique to label and visualize the cell membrane's molecules.
Trypan blue is an azo dye.It is a direct dye for cotton textiles. [3] In biosciences, it is used as a vital stain to selectively colour dead tissues or cells blue.. Live cells or tissues with intact cell membranes are not coloured.
Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Note: TTC is somewhat heat and light unstable, so avoid these environments as much as possible.