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The suction device must maintain a pressure of -140 mmHg (necessary to aspirate rapidly, but not enough to damage the follicles) and a temperature of approximately 37 °C. The follicular fluid is delivered to a technician in the IVF laboratory to identify and quantify the ova.
Controlled ovarian hyperstimulation, which is generally part of in vitro fertilization, and the aim is generally to develop multiple follicles (optimally between 11 and 14 antral follicles measuring 2–8 mm in diameter), [medical citation needed] followed by transvaginal oocyte retrieval, co-incubation, followed by embryo transfer of a maximum ...
In vitro fertilisation (IVF) is a process of fertilisation in which an egg is combined with sperm in vitro ("in glass"). The process involves monitoring and stimulating a woman's ovulatory process, then removing an ovum or ova (egg or eggs) from her ovaries and enabling a man's sperm to fertilise them in a culture medium in a laboratory.
Recently, two publications have challenged the idea that a finite number of follicles are set around the time of birth. [11] [12] Renewal of ovarian follicles from germline stem cells (originating from bone marrow and peripheral blood) was reported in the postnatal mouse ovary. Studies attempting to replicate these results are underway, but a ...
The ovary is generally thought of as an egg bank from which the woman draws during her reproductive life. The human ovary contains a population of primordial follicles.At 18–22 weeks post-conception, the female ovary contains its peak number of follicles (about 300,000 in the average case, but individual peak populations range from 35,000 to 2.5 million [3]). p The size of the initial ...
In vitro maturation (IVM) is the technique of letting the contents of ovarian follicles and the oocytes inside mature in vitro. It can be offered to women with infertility problems, combined with In Vitro Fertilization (IVF), offering women pregnancy without ovarian stimulation.
In the ovaries, immature eggs are housed in fluid-filled sacs called follicles. When an egg matures, the follicle ruptures and releases the egg during ovulation.
Cell culture of granulosa cells can be performed in vitro. Plating density (number of cells per volume of culture medium) plays a critical role for the differentiation. A lower plating density makes granulosa cells exhibit estrogen production, while a higher plating density makes them appear as progesterone producing theca lutein cells. [12]