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N-Ethylmaleimide (NEM) is an organic compound that is derived from maleic acid. It contains the amide functional group , but more importantly it is an alkene that is reactive toward thiols and is commonly used to modify cysteine residues in proteins and peptides .
In chemistry, the molar mass (M) (sometimes called molecular weight or formula weight, but see related quantities for usage) of a chemical compound is defined as the ratio between the mass and the amount of substance (measured in moles) of any sample of the compound. [1] The molar mass is a bulk, not molecular, property of a substance.
The molar mass is defined as the mass of a given substance divided by the amount of the substance, and is expressed in grams per mol (g/mol). That makes the molar mass an average of many particles or molecules (potentially containing different isotopes), and the molecular mass the mass of one specific particle or molecule. The molar mass is ...
The mass average molar mass (often loosely termed weight average molar mass) is another way of describing the molar mass of a polymer. Some properties are dependent on molecular size, so a larger molecule will have a larger contribution than a smaller molecule.
The Dumas method of molecular weight determination was historically a procedure used to determine the molecular weight of an unknown volatile substance. [ 1 ] [ 2 ] The method was designed by the French chemist Jean Baptiste André Dumas , after whom the procedure is now named.
Historically, the mole was defined as the amount of substance in 12 grams of the carbon-12 isotope.As a consequence, the mass of one mole of a chemical compound, in grams, is numerically equal (for all practical purposes) to the mass of one molecule or formula unit of the compound, in daltons, and the molar mass of an isotope in grams per mole is approximately equal to the mass number ...
Mass fraction can also be expressed, with a denominator of 100, as percentage by mass (in commercial contexts often called percentage by weight, abbreviated wt.% or % w/w; see mass versus weight). It is one way of expressing the composition of a mixture in a dimensionless size ; mole fraction (percentage by moles , mol%) and volume fraction ...
Maleimide-mediated methodologies are among the most used in bioconjugation. [5] [6] Due to fast reactions and high selectivity towards cysteine residues in proteins, a large variety of maleimide heterobifunctional reagents are used for the preparation of targeted therapeutics, assemblies for studying proteins in their biological context, protein-based microarrays, or proteins immobilisation. [7]