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In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.
Calculus was a term used for various kinds of stones. This spun off many modern words, including calculate (' use stones for mathematical purposes '), and calculus, which came to be used, in the 18th century, for accidental or incidental mineral buildups in human and animal bodies, like kidney stones and minerals on teeth. [3]
ZOE can be used as a dental filling material or dental cement in dentistry. [ 1 ] [ 2 ] It is often used in dentistry when the decay is very deep or very close to the nerve or pulp chamber. Because the tissue inside the tooth, i.e. the pulp , reacts badly to the drilling stimulus (heat and vibration), it frequently becomes severely inflamed and ...
Unlike negative staining, positive staining uses basic dyes to color the specimen against a bright background. While chromophore is used for both negative and positive staining alike, the type of chromophore used in this technique is a positively charged ion instead of a negative one. The negatively charged cell wall of many microorganisms ...
1. Isolate the tooth with a rubber dam 2. Perform a pulpotomy procedure. 3. Place the MTA material over the pulp and close the tooth with temporary cement until the apex is completely formed. MTA can be used in a one step or a two step approach. It can be used as a powder or a Wet Mix.
The bath consists of three containers filled with water at different temperatures: the first is set at 100 °C to liquefy the agar, the second is used to lower down the temperature of the material for safe intra-oral use (usually set at 43–46 °C) and the third one is used for storage and is set at 63–66 °C.
[1] [2] [3] The Diff-Quik procedure is based on a modification of the Wright-Giemsa stain pioneered by Harleco in the 1970s, [1] and has advantages over the routine Wright-Giemsa staining technique in that it reduces the 4-minute process into a much shorter operation and allows for selective increased eosinophilic or basophilic staining ...
Molar incisor hypomineralisation (MIH) is a type of enamel defect affecting, as the name suggests, the first molars and incisors in the permanent dentition. [1] MIH is considered a worldwide problem with a global prevalence of 12.9% and is usually identified in children under 10 years old. [2]