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In molecular biology, the five-prime cap (5′ cap) is a specially altered nucleotide on the 5′ end of some primary transcripts such as precursor messenger RNA.This process, known as mRNA capping, is highly regulated and vital in the creation of stable and mature messenger RNA able to undergo translation during protein synthesis.
The NAD+ 5' cap has been observed in bacteria, [3] contrary to the long-held belief that prokaryotes lacked 5'-capped RNA, [4] as well as on the 5' cap of eukaryotic mRNA, [5] in place of the m7G cap. This modification also potentially allows for selective degradation of RNA]within prokaryotes as different pathways are involved in the ...
It consists of a five-prime cap; a five prime untranslated region derived from the sequence of human alpha globin; a signal peptide (bases 55–102) and two proline substitutions (K986P and V987P, designated "2P") that cause the spike to adopt a prefusion-stabilized conformation reducing the membrane fusion ability, increasing expression and ...
The CHMP recommends authorizing Moderna's (MRNA) bivalent COVID-19 booster vaccine, targeting the Omicron BA.4/5 subvariants, for use in adults and adolescents aged 12 years and older.
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Following lab tests, Moderna said its current version of the vaccine would continue to be its "first line of defense against Omicron."
Conventional messenger RNA (mRNA) vaccines only produce a finite amount of protein due to the short mRNA half-life. saRNA extends the kinetics of expression by a second ORF encoding the protein machinery necessary for its own replication. This self-replication dramatically increases both the amount of RNA and the time of expression.
The 5′ untranslated region (also known as 5′ UTR, leader sequence, transcript leader, or leader RNA) is the region of a messenger RNA (mRNA) that is directly upstream from the initiation codon. This region is important for the regulation of translation of a transcript by differing mechanisms in viruses , prokaryotes and eukaryotes .
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