Search results
Results from the WOW.Com Content Network
When a cell divides, the double helix of DNA is divided, as well. Centromeres coordinate the precise, nearly perfect matchmaking of the separated strands with their associated enzymes in each new ...
A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or characteristics which must be defined. Cell strains are cells that have been adapted to culture but, unlike cell lines, have a finite division potential.
The proteolysis of heterologously expressed proteins is reduced due to the functional deficiency of two major proteases, Lon and OmpT. [3] Lon is usually present in the cytoplasm of the cell, but in all B strains its production is prevented by an insertion within the promoter sequence.
The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. [2]In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to ...
Genotype of cells – Cloning strains may contain mutations that improve the transformation efficiency of the cells. For example, E. coli K12 strains with the deoR mutation, originally found to confer an ability of cell to grow in minimum media using inosine as the sole carbon source, have 4-5 times the transformation efficiency of similar ...
The Hayflick limit, or Hayflick phenomenon, is the number of times a normal somatic, differentiated human cell population will divide before cell division stops. [ 1 ] [ 2 ] The concept of the Hayflick limit was advanced by American anatomist Leonard Hayflick in 1961, [ 3 ] at the Wistar Institute in Philadelphia , Pennsylvania.
In order to verify that Esp1 does play a role in regulating Scc1 chromosome association, cell strains were arrested in G1 with an alpha factor. These cells stayed in arrest during the development. Esp1-1 mutant cells were used and the experiment was repeated, and Scc1 successfully bound to the chromosomes and remained associated even after the ...
Get AOL Mail for FREE! Manage your email like never before with travel, photo & document views. Personalize your inbox with themes & tabs. You've Got Mail!