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The Lineweaver–Burk plot derives from a transformation of the Michaelis–Menten equation, = + in which the rate is a function of the substrate concentration and two parameters , the limiting rate, and , the Michaelis constant.
In enzyme kinetics, a secondary plot uses the intercept or slope from several Lineweaver–Burk plots to find additional kinetic constants. [1] [2]For example, when a set of v by [S] curves from an enzyme with a ping–pong mechanism (varying substrate A, fixed substrate B) are plotted in a Lineweaver–Burk plot, a set of parallel lines will be produced.
A decade before Michaelis and Menten, Victor Henri found that enzyme reactions could be explained by assuming a binding interaction between the enzyme and the substrate. [11] His work was taken up by Michaelis and Menten, who investigated the kinetics of invertase , an enzyme that catalyzes the hydrolysis of sucrose into glucose and fructose ...
On a Lineweaver-Burk plot, the presence of a noncompetitive inhibitor is illustrated by a change in the y-intercept, defined as 1/V max. The x-intercept, defined as −1/K M, will remain the same. In competitive inhibition, the inhibitor will bind to an enzyme at the active site, competing with the substrate.
The plot is occasionally attributed to Augustinsson [5] and referred to the Woolf–Augustinsson–Hofstee plot [6] [7] [8] or simply the Augustinsson plot. [9] However, although Haldane, Woolf or Eadie were not explicitly cited when Augustinsson introduced the versus / equation, both the work of Haldane [10] and of Eadie [3] are cited at other places of his work and are listed in his ...
Of the seven players who have found new teams, four are now searching for lodging in Orange County, California. That’s because no team in baseball has been more active so far this winter than ...
Get The Recipe. Why You Should Be Making Gumbo With Leftover Turkey. While Thanksgiving is a kind of whirlwind of cooking, gumbo is a slow process—one you might appreciate at the end of the busy ...
Strength training. She combines all that swimming with strength training.She says that she gets in the weight room twice a week for 30 minutes each time, with a primary focus on her shoulders and ...