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2. Combined bisulfite restriction analysis - Wikipedia

   en.wikipedia.org/wiki/Combined_Bisulfite...

   The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites. Combined Bisulfite Restriction Analysis (or COBRA) is a molecular biology technique that allows for the sensitive quantification of DNA methylation levels at a specific genomic locus on a DNA sequence in a small sample of genomic DNA. [1]

3. Bayesian tool for methylation analysis - Wikipedia

   en.wikipedia.org/wiki/Bayesian_tool_for...

   Bayesian tool for methylation analysis, also known as BATMAN, is a statistical tool for analysing methylated DNA immunoprecipitation (MeDIP) profiles. It can be applied to large datasets generated using either oligonucleotide arrays (MeDIP-chip) or next-generation sequencing (MeDIP-seq), providing a quantitative estimation of absolute methylation state in a region of interest.

4. Circulating tumor DNA - Wikipedia

   en.wikipedia.org/wiki/Circulating_tumor_DNA

   A normal epigenetic status is maintained in a cell at least in part through DNA methylation. [31] Measuring aberrant methylation patterns in ctDNA is possible due to stable methylation of regions of DNA referred to as “CpG islands”. Methylation of ctDNA can be detected through bisulfite treatment. Bisulfite treatment chemically converts ...

5. Bisulfite sequencing - Wikipedia

   en.wikipedia.org/wiki/Bisulfite_sequencing

   Bisulfite sequencing applies routine sequencing methods on bisulfite-treated genomic DNA to determine methylation status at CpG dinucleotides. Other non-sequencing strategies are also employed to interrogate the methylation at specific loci or at a genome-wide level. All strategies assume that bisulfite-induced conversion of unmethylated ...

6. High-resolution melting analysis - Wikipedia

   en.wikipedia.org/wiki/High-resolution_melting...

   In a study which tested the methylation status of the MGMT promoter on 19 colorectal samples, 8 samples were found to be methylated. [8] Another study compared the predictive power of MGMT promoter methylation in 83 high grade glioma patients obtained by either MSP, pyrosequencing, and HRM. The HRM method was found to be at least equivalent to ...

7. NOMe-seq - Wikipedia

   en.wikipedia.org/wiki/NOMe-seq

   NOMe-seq has the additional benefit of providing DNA methylation status, which also plays a crucial role in the regulation of genomic activity. Interestingly, increased DNA methylation is associated with transcriptional silencing whereas accessible DNA unbound by nucleosomes is generally associated with transcriptional activation. In this sense ...

8. Reduced representation bisulfite sequencing - Wikipedia

   en.wikipedia.org/wiki/Reduced_representation_bi...

   Enzyme digestion: First, genomic DNA is digested using a methylation-insensitive restriction enzyme. It is integral for the enzymes to not be influenced by the methylation status of the CpGs (sites within the genome where a cytosine is next to a guanine) as this allows for the digestion of both methylated and unmethylated areas.

9. Whole genome bisulfite sequencing - Wikipedia

   en.wikipedia.org/wiki/Whole_genome_bisulfite...

   Whole genome bisulfite sequencing is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating the DNA with sodium bisulfite before high-throughput DNA sequencing. The DNA methylation status at various genes can reveal information regarding gene regulation and transcriptional activities ...