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Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2] Gram ...
Gram-negative bacteria are bacteria that, unlike gram-positive bacteria, do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. [1] Their defining characteristic is their cell envelope , which consists of a thin peptidoglycan cell wall sandwiched between an inner ( cytoplasmic ) membrane and an ...
Haemophilus influenzae (formerly called Pfeiffer's bacillus or Bacillus influenzae) is a Gram-negative, non-motile, coccobacillary, facultatively anaerobic, capnophilic pathogenic bacterium of the family Pasteurellaceae. The bacteria are mesophilic and grow best at temperatures between 35 and 37 °C. [1]
Fusobacterium is a genus of obligate anaerobic, Gram-negative, [2] non-sporeforming bacteria [3] belonging to Gracilicutes. Individual cells are slender, rod-shaped bacilli with pointed ends. [ 4 ] [ 5 ] Fusobacterium was discovered in 1900 by Courmont and Cade and is common in the flora of humans.
Proteus mirabilis appears as Gram-negative rods after Gram staining under bright-field microscopy with 1000 times magnification. Proteus mirabilis is a Gram-negative, facultatively anaerobic, rod-shaped bacterium. It shows swarming motility and urease activity. P. mirabilis causes 90% of all Proteus infections in humans.
Enterobacter is a genus of common Gram-negative, facultatively anaerobic, rod-shaped, non-spore-forming bacteria of the family Enterobacteriaceae. Cultures are found in soil, water, sewage, feces and gut environments. It is the type genus of the order Enterobacterales. [1]
Brucella is a genus of Gram-negative bacteria, [2] [3] named after David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 μm), ... On Gram stain, they ...
The Gram stain, developed in 1884 by Hans Christian Gram, characterises bacteria based on the structural characteristics of their cell walls. [174] [75] The thick layers of peptidoglycan in the "Gram-positive" cell wall stain purple, while the thin "Gram-negative" cell wall appears pink. [174]