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  2. Guanidinium thiocyanate - Wikipedia

    en.wikipedia.org/wiki/Guanidinium_thiocyanate

    A commonly used method is guanidinium thiocyanate-phenol-chloroform extraction. It is not strictly necessary to use phenol or chloroform if extracting RNA for Northern blotting or DNA for Southern blot analysis because the gel electrophoresis followed by transfer to a membrane will separate the RNA/DNA from the proteins. Additionally, since ...

  3. Trizol - Wikipedia

    en.wikipedia.org/wiki/Trizol

    The correct name of the method is guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield. [5] [6] An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with ...

  4. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology. It is widely used for isolating RNA (as well as DNA and protein in some cases).

  5. RNA extraction - Wikipedia

    en.wikipedia.org/wiki/RNA_extraction

    Several methods are used in molecular biology to isolate RNA from samples, the most common of these is guanidinium thiocyanate-phenol-chloroform extraction. [2] [3] The filter paper based lysis and elution method features high throughput capacity. [4]

  6. Boom method - Wikipedia

    en.wikipedia.org/wiki/Boom_method

    According to the original Boom method, the chaotropic guanidinium salt employed is preferably guanidinium thiocyanate (GuSCN). According to the chaotropic effect, in the presence of the chaotropic agent, hydration water of nucleic acids are taken from the phosphodiester bond of the phosphate group of the backbone of a nucleic acid.

  7. Nicoletta Sacchi - Wikipedia

    en.wikipedia.org/wiki/Nicoletta_Sacchi

    Nicoletta Sacchi (born August 20, 1949) is an Italian professor of oncology at the Roswell Park Comprehensive Cancer Center. [1] She is the co-discoverer of the acid guanidinium thiocyanate-phenol-chloroform extraction method to extract RNA from biological samples with Trizol. [2]

  8. Small RNA sequencing - Wikipedia

    en.wikipedia.org/wiki/Small_RNA_sequencing

    acid guanidinium thiocyanate-phenol-chloroform extraction: it is based on the use of a guanidinium-thiocyanate solution combined with acid phenol that disrupts cell membranes bringing in solution the nucleic acids and inactivating cellular ribonucleases (chaotropic agent). After this step an aliquot of chloroform is added in order to separate ...

  9. Phenol–chloroform extraction - Wikipedia

    en.wikipedia.org/wiki/Phenol–chloroform_extraction

    This mixture is then centrifuged. Because the phenol:chloroform mixture is immiscible with water, the centrifuge will cause two distinct phases to form: an upper aqueous phase, and a lower organic phase. The aqueous phase rises to the top because it is less dense than the organic phase containing the phenol:chloroform.

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