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The labeled salt bridge shows the U-shaped glass tube used as a salt bridge. To set up a glass tube salt bridge, a U-shaped Vycor tube is fashioned to contain a suitable electrolyte solution. [ 3 ] Normally, glass frits, a porous material, cover the ends of the tube or the electrolyte is often gelified with agar-agar to help prevent the ...
To maintain the salt bridge, His31 will attempt to keep its proton as long as possible. When the salt bridge is disrupted, like in the mutant D70N, the pK a shifts back to a value of 6.9, much closer to that of His31 in the unfolded state. The difference in pK a can be quantified to reflect the salt bridge’s contribution to free energy.
The salt bridge may be replaced periodically but contamination of the other electrolytes will persist. How is this handles if for example measurements require a the use of a salt bridge over extended periods? It would be nice if somebody could treat such aspects in the article.150.227.15.253 09:26, 20 May 2020 (UTC)
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Hashiwokakero (橋をかけろ Hashi o kakero; lit. "build bridges!") is a type of logic puzzle published by Nikoli. [1] It has also been published in English under the name Bridges or Chopsticks (based on a mistranslation: the hashi of the title, 橋, means bridge; hashi written with another character, 箸, means chopsticks).
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Example salt bridge network in 2-deoxyribose-5-phosphate aldolase ( ). Interactions are shown as cyan dashed lines. Polar residues are colored green. Polar amino acids aspartate (D), glutamate (E), lysine (K), and arginine (R), are shown here. Salt bridges within TIM barrel pores are thought to contribute to the overall stability of the fold.
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