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In genetics, a three-point cross is used to determine the loci of three genes in an organism's genome.. An individual heterozygous for three mutations is crossed with a homozygous recessive individual, and the phenotypes of the progeny are scored.
The three-point bending flexural test provides values for the modulus of elasticity in bending, flexural stress, flexural strain and the flexural stress–strain response of the material. This test is performed on a universal testing machine (tensile testing machine or tensile tester) with a three-point or four-point bend fixture.
The transverse bending test is most frequently employed, in which a specimen having either a circular or rectangular cross-section is bent until fracture or yielding using a three-point flexural test technique. The flexural strength represents the highest stress experienced within the material at its moment of yield.
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The first uses of test crosses were in Gregor Mendel’s experiments in plant hybridization.While studying the inheritance of dominant and recessive traits in pea plants, he explains that the “signification” (now termed zygosity) of an individual for a dominant trait is determined by the expression patterns of the following generation.
The forked-line method (also known as the tree method and the branching system) can also solve dihybrid and multi-hybrid crosses. A problem is converted to a series of monohybrid crosses, and the results are combined in a tree. However, a tree produces the same result as a Punnett square in less time and with more clarity.
For example, a triangular distribution might be used, depending on the application. In three-point estimation, three figures are produced initially for every distribution that is required, based on prior experience or best-guesses: a = the best-case estimate; m = the most likely estimate; b = the worst-case estimate
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