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Auxotrophic genetic markers are often used in molecular genetics; they were famously used in Beadle and Tatum's Nobel prize-winning work on the one gene-one enzyme hypothesis, connecting mutations of genes to protein mutations. This then allows for biosynthetic or biochemical pathway mapping that can help determine which enzyme or enzymes are ...
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
The mixture of treated cells is cultured on media that contain the antibiotic so that only transformed cells are able to grow. Another method of selection is the use of certain auxotrophic markers that can compensate for an inability to metabolise certain amino acids, nucleotides, or sugars. This method requires the use of suitably mutated ...
Auxotrophic selection markers that allow an auxotrophic organism to grow in minimal growth medium may also be used; examples of these are LEU2 and URA3 which are used with their corresponding auxotrophic strains of yeast. [7] Another kind of selectable marker allows for the positive selection of plasmid with cloned gene.
Ames test procedure. The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenic potential of chemical compounds. [1]
In biomedical contexts, a biomarker, or biological marker, is a measurable indicator of some biological state or condition. Biomarkers are often measured and evaluated using blood, urine, or soft tissues [ 1 ] to examine normal biological processes , pathogenic processes, or pharmacologic responses to a therapeutic intervention . [ 2 ]
Cardiac markers are biomarkers measured to evaluate heart function. They can be useful in the early prediction or diagnosis of disease. [ 1 ] Although they are often discussed in the context of myocardial infarction , other conditions can lead to an elevation in cardiac marker level.
17117 Ensembl ENSG00000242110 ENSMUSG00000022244 UniProt Q9UHK6 O09174 RefSeq (mRNA) NM_203382 NM_001167595 NM_001167596 NM_001167597 NM_001167598 NM_014324 NM_008537 RefSeq (protein) NP_001161067 NP_055139 NP_976316 NP_032563 Location (UCSC) Chr 5: 33.99 – 34.01 Mb Chr 15: 10.98 – 11 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse α-Methylacyl-CoA racemase (AMACR, EC 5.1.99.4 ...