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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain, but other organisms such as yeast may also be used. DNA of transformation is ligated into a ...
The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000, etc.) in sterile water and cultivating these on nutrient agar in a dish that is sealed and incubated. Typical media include plate count agar for a general count or MacConkey agar to count Gram-negative bacteria such as E. coli. Typically one set of ...
Anaerobic bacteria collection can come from a variety of sources in patient samples, including blood, bile, bone marrow, cerebrospinal fluid, direct lung aspirate, tissue biopsies from a normally sterile site, fluid from a normally sterile site (like a joint), dental, abscess, abdominal or pelvic abscess, knife, gunshot, or surgical wound, or ...
All aspects of the Kirby–Bauer procedure are standardized to ensure consistent and accurate results. Because of this, a laboratory must adhere to these standards. The media used in Kirby–Bauer testing must be Mueller–Hinton agar at only 4 mm deep, poured into either 100 mm or 150 mm Petri dishes.
Microtox is an in vitro testing system which uses bioluminescent bacteria (Allivibrio fischeri, formerly known as Vibrio fischeri) to detect toxic substances in different substrates such as water, air, soils and sediments. [1] Allivibrio fischeri are non-pathogenic, marine, bacteria that luminesce as a natural part of their metabolism. [2]
The CAMP test (Christie–Atkins–Munch-Petersen) is a test to identify group B β-hemolytic streptococci (Streptococcus agalactiae) [1] [2] based on their formation of a substance, CAMP factor, [3] that enlarges the area of hemolysis formed by the β-hemolysin elaborated from Staphylococcus aureus.
Bacteria are not able to grow around antibiotics to which they are sensitive. This is called "the zone of inhibition". Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics. It is used because bacteria may have resistance to some antibiotics.
The acidity of the dyes causes them to bind more strongly to the cell walls of the bacteria than to other cells or tissues. This results in the selective staining of only those cells that have a high density of cell wall material, such as acid-fast bacteria. [27] The Ziehl-Neelsen stain is a two step staining process. In the first step, the ...