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Cell-free protein synthesis, also known as in vitro protein synthesis or CFPS, is the production of protein using biological machinery in a cell-free system, that is, without the use of living cells. The in vitro protein synthesis environment is not constrained by a cell wall or homeostasis conditions necessary to maintain cell viability. [ 1 ]
A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full cell system, thus reducing the complex interactions typically found when working in a whole cell. [1]
Systems biology is the computational and mathematical analysis and modeling of complex biological systems.It is a biology-based interdisciplinary field of study that focuses on complex interactions within biological systems, using a holistic approach (holism instead of the more traditional reductionism) to biological research.
Holism in science, holistic science, or methodological holism is an approach to research that emphasizes the study of complex systems.Systems are approached as coherent wholes whose component parts are best understood in context and in relation to both each other and to the whole.
Holism is the interdisciplinary idea that systems possess properties as wholes apart from the properties of their component parts. [1] [2] [3] The aphorism "The whole is greater than the sum of its parts", typically attributed to Aristotle, is often given as a summary of this proposal. [4]
It is a holistic, economic, industrial and social framework that seeks to create systems that are not only efficient but also essentially waste free. [2] Building off the whole systems approach of John T. Lyle's regenerative design, the model in its broadest sense is not limited to industrial design and manufacturing; it can be applied to many ...
An even more radical change in the genetic code is the change of a triplet codon to a quadruplet and even quintuplet codon pioneered by Sisido in cell-free systems [47] and by Schultz in bacteria. [48] Finally, non-natural base pairs can be used to introduce novel amino acid in proteins. [49]
After identifying the need for reform in the fundamental concepts of matter, life, and mind (chapter 1), Smuts examines the reformed concepts (as of 1926) of space and time (chapter 2), matter (chapter 3), and biology (chapter 4), and concludes that the close approach to each other of the concepts of matter, life, and mind, and the partial overflow of each other's domains, imply that there is ...