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A light microscope is a biology laboratory instrument or tool, that uses visible light to detect and magnify very small objects and enlarge them. They use lenses to focus light on the specimen, magnifying it thus producing an image. The specimen is normally placed close to the microscopic lens.
In a simple light microscope, a thin specimen containing a slide is placed on the microscope’s stage. A beam of light is passed through the condenser to the specimen. The light transmitted from the specimen enters the objective lens.
Review the principles of light microscopy and identify the major parts of the microscope. Learn how to use the microscope to view slides of several different cell types, including the use of the oil immersion lens to view bacterial cells.
Light microscopes can reveal the structures of living cells and tissues, as well as of non-living samples such as rocks and semiconductors. Microscopes can be simple or complex in design, and some can do more than one type of microscopy, each of which reveals slightly different information.
Light microscopes are used to examine cells at relatively low magnifications. Magnifications of about 2000X are the upper limit for light microscopes. The highest resolution of a light microscope is about 0.2 μm. The use of blue light to illuminate a specimen gives the highest resolution.
A light microscope is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects whereas electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination.
The Basics. Diagram of a typical student light microscope, showing the parts and the light path. A light microscope works very much like a refracting telescope, but with some minor differences. Let's briefly review how a telescope works.
Microscopes are effectively just tubes packed with lenses, curved pieces of glass that bend (or refract) light rays passing through them. The simplest microscope of all is a magnifying glass made from a single convex lens, which typically magnifies by about 5–10 times.
All of the parts of a microscope work together - The light from the illuminator passes through the aperture, through the slide, and through the objective lens, where the image of the specimen is magnified.
Made of several parts such as the eyepiece, objective lenses, diaphragm, and stage, they use glass elements to reflect and warp light, creating a magnified image that can be seen with the human eye. The light source may be indirect through a mirror, or directly with a light illuminator.