Search results
Results from the WOW.Com Content Network
A DNase footprinting assay [1] is a DNA footprinting technique from molecular biology/biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage. This makes it possible to locate a protein binding site on a particular DNA molecule.
In vivo footprinting is a technique used to analyze the protein-DNA interactions that are occurring in a cell at a given time point. [5] [9] DNase I can be used as a cleavage agent if the cellular membrane has been permeabilized. However the most common cleavage agent used is UV irradiation because it penetrates the cell membrane without ...
DNase is commonly used when purifying proteins that are extracted from prokaryotic organisms. Protein extraction often involves the degradation of the cell membrane . It is common for the degraded and fragile cell membrane to be lysed , releasing unwanted DNA and the desired proteins.
Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...
The first known deoxyribozyme was a ribonuclease, discovered in 1994 by Ronald Breaker while a postdoctoral fellow in the laboratory of Gerald Joyce at the Scripps Research Institute. [9] This deoxyribozyme, later named GR-5, [ 10 ] catalyzes the Pb 2+ -dependent cleavage of a single ribonucleotide phosphoester at a rate that is more than 100 ...
The study of DHS profiles combined with other techniques allows analysis of regulatory DNA in humans: Transcription factor: Using the ChIP-Seq technique, the binding sites to DNA in certain transcription factor groups are determined, and the DHS profiles are compared. The results confirm a high correlation, which show that the coordinated union ...
Radioactive sulfur-35 was used to label the protein sections of the T2 phage, because sulfur is contained in protein but not DNA. [ 6 ] Hershey and Chase inserted the radioactive elements in the bacteriophages by adding the isotopes to separate media within which bacteria were allowed to grow for 4 hours before bacteriophage introduction.
A polymerase chain reaction is a form of enzymatic DNA synthesis in the laboratory, using cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. DNA synthesis during PCR is very similar to living cells but has very specific reagents and conditions.