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The traditional manual method to patch clamp using glass pipettes was developed by Erwin Neher and Bert Sakmann and required a highly skilled technician. The technician would position the glass pipette near a cell and apply the appropriate suction to create an electrical seal between the pipette and the cell membrane.
Toro (登呂 遺跡, Toro iseki) is an archaeological site in Suruga Ward in Shizuoka City, 130 kilometres (81 mi) southwest of Tokyo, Japan.The site contains the ruins of a settlement which dates to the 1st century CE, in the late Yayoi period.
Classical patch clamp setup, with microscope, antivibration table, and micromanipulators. During a patch clamp recording, a hollow glass tube known as a micropipette or patch pipette filled with an electrolyte solution and a recording electrode connected to an amplifier is brought into contact with the membrane of an isolated cell.
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The A3000 series trains operate on the 11.0 km (6.8 mi) Shizuoka Railway Shizuoka–Shimizu Line in Shizuoka Prefecture, which runs between Shin-Shizuoka and Shin-Shimizu. A total of twelve two-car trains are scheduled to be introduced over a period of eight years, replacing the company's older 1000 series trains. [1]
In the Meiji period from 1868 to 1871, the title of the Shizuoka daimyō was han-chiji or chihanji (domainal governor). [3] In 1871, Shizuoka Domain was replaced by Shizuoka Prefecture. [4] The lands of the former Shizuoka Domain now form the western two-thirds of Shizuoka Prefecture, plus the Chita Peninsula in Aichi Prefecture.
From better fast-charging for long drives in electric vehicles to powered climate control vents, 2024’s best new-car features came in some surprising packages.