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Cold Spring Harbor Protocols (formerly CSH Protocols) is an online scientific journal and methods database for biologists, published by Cold Spring Harbor Laboratory Press. Protocols are presented step-by-step and edited in the style that has made Molecular Cloning, Antibodies, Cells and many other CSH manuals essential [ tone ] to the work of ...
He did postdoctoral research at the MRC Laboratory of Molecular Biology (1966–67) and the Salk Institute for Biological Studies (1967–69). In 1969 he was hired by James D. Watson to work at the Cold Spring Harbor Laboratory in New York. Watson has been reported to say this was the best hiring decision he ever made.
While an assistant professor of Biochemistry and Molecular Biology at Harvard, and a member of the Cold Spring Harbor Laboratory (CSHL) faculty, Maniatis collaborated with Drs. Fotis Kafatos and Argiris Efstratiadis to develop a method for synthesizing and cloning full length double stranded DNA copies of messenger RNA (termed “copy” or cDNA).
To reduce the number of mutations in the PCR product (e.g. for molecular cloning), ... Cold Spring Harbor Laboratory Press; 3rd edition (2001), ISBN 0-87969-577-3.
Cold Spring Harbor Laboratory (CSHL) is a private, non-profit institution with research programs focusing on cancer, neuroscience, plant biology, genomics, and quantitative biology. [2] It is located in Laurel Hollow on Long Island , New York.
Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...
There are two fundamental differences between the methods. One is that molecular cloning involves replication of the DNA within a living cell, while PCR replicates DNA in the test tube, free of living cells. The other difference is that cloning involves cutting and pasting DNA sequences, while PCR amplifies by copying an existing sequence.
[3] [4] In 1982, Fritsch, Joe Sambrook, and Maniatis wrote Molecular Cloning: A Laboratory Manual, which was considered "omnipresent in Molecular Biology laboratories and [...] utilized to the point where it is frequently referred to as ‘The Bible’.” [5] Fritsch helped initiate and for four years co-taught the widely successful Cold ...