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Cold Spring Harbor Protocols (formerly CSH Protocols) is an online scientific journal and methods database for biologists, published by Cold Spring Harbor Laboratory Press. Protocols are presented step-by-step and edited in the style that has made Molecular Cloning, Antibodies, Cells and many other CSH manuals essential [ tone ] to the work of ...
While an assistant professor of Biochemistry and Molecular Biology at Harvard, and a member of the Cold Spring Harbor Laboratory (CSHL) faculty, Maniatis collaborated with Drs. Fotis Kafatos and Argiris Efstratiadis to develop a method for synthesizing and cloning full length double stranded DNA copies of messenger RNA (termed “copy” or cDNA).
Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...
There are two fundamental differences between the methods. One is that molecular cloning involves replication of the DNA within a living cell, while PCR replicates DNA in the test tube, free of living cells. The other difference is that cloning involves cutting and pasting DNA sequences, while PCR amplifies by copying an existing sequence.
It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components. The purified DNA can then be used for downstream applications such as PCR, [2] sequencing, or cloning. Currently, it is a routine procedure in molecular biology or forensic analyses.
Edward Francis Fritsch (born June 1, 1950) is a scientist in the field of molecular biology and cancer immunology. [1] Fritsch completed his PhD at the University of Wisconsin–Madison under Howard Temin. [1] [2] His thesis was titled, "Synthesis and structure of avian retrovirus DNA". [1]
Cold Spring Harbor Laboratory (CSHL) is a private, non-profit institution with research programs focusing on cancer, neuroscience, plant biology, genomics, and quantitative biology. [2] It is located in Laurel Hollow on Long Island , New York.
Molecular weight 80kDa [1] 94kDa [1] 94kDa [1] ... Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press; 3rd edition ...