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  2. Equivalence point - Wikipedia

    en.wikipedia.org/wiki/Equivalence_point

    Different methods to determine the equivalence point include: pH indicator A pH indicator is a substance that changes color in response to a chemical change. An acid-base indicator (e.g., phenolphthalein) changes color depending on the pH. Redox indicators are also frequently used. A drop of indicator solution is added to the titration at the ...

  3. Titration curve - Wikipedia

    en.wikipedia.org/wiki/Titration_curve

    Halfway between each equivalence point, at 7.5 mL and 22.5 mL, the pH observed was about 1.5 and 4, giving the pK a. In weak monoprotic acids , the point halfway between the beginning of the curve (before any titrant has been added) and the equivalence point is significant: at that point, the concentrations of the two species (the acid and ...

  4. pH indicator - Wikipedia

    en.wikipedia.org/wiki/PH_indicator

    The equations, derived from the acidity constant and basicity constant, states that when pH equals the pK a or pK b value of the indicator, both species are present in a 1:1 ratio. If pH is above the p K a or p K b value, the concentration of the conjugate base is greater than the concentration of the acid, and the color associated with the ...

  5. Acid–base titration - Wikipedia

    en.wikipedia.org/wiki/Acid–base_titration

    pH after the equivalence point; 1. The initial pH is approximated for a weak acid solution in water using the equation: [1] = ⁡ [+] where [+] is the initial concentration of the hydronium ion. 2. The pH before the equivalence point depends on the amount of weak acid remaining and the amount of conjugate base formed.

  6. Gran plot - Wikipedia

    en.wikipedia.org/wiki/Gran_plot

    For a strong acid-strong base titration monitored by pH, we have at any i'th point in the titration = [+] [] where K w is the water autoprotolysis constant.. If titrating an acid of initial volume and concentration [+] with base of concentration [], then at any i'th point in the titration with titrant volume ,

  7. Protein pKa calculations - Wikipedia

    en.wikipedia.org/wiki/Protein_pKa_calculations

    From the titration of protonatable group, one can read the so-called pK a 1 ⁄ 2 which is equal to the pH value where the group is half-protonated (i.e. when 50% such groups would be protonated). The pK a 1 ⁄ 2 is equal to the Henderson–Hasselbalch pK a (pK HH a) if the titration curve follows the Henderson–Hasselbalch equation. [14]

  8. Isoelectric point - Wikipedia

    en.wikipedia.org/wiki/Isoelectric_point

    The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. The standard nomenclature to represent the isoelectric point is pH(I). [1] However, pI is also used. [2] For brevity, this article uses pI.

  9. Predominance diagram - Wikipedia

    en.wikipedia.org/wiki/Predominance_diagram

    The three species all have concentrations equal to ⁠ 1 / K D ⁠ at pH = pK 1, for which [Cr] = ⁠ 4 / K D ⁠. [3] The three lines on this diagram meet at that point. Green line Chromate and hydrogen chromate have equal concentrations. Setting [CrO 2− 4] equal to [HCrO − 4] in eq. 1, [H +] = ⁠ 1 / K 1 ⁠, or pH = log K 1. This ...