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The theory of high performance liquid chromatography-HPLC is, at its core, the same as general chromatography theory. [41] This theory has been used as the basis for system-suitability tests, as can be seen in the USP Pharmacopeia, [ 42 ] which are a set of quantitative criteria, which test the suitability of the HPLC system to the required ...
In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.
Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography.
A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.
Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
Another example of vapor and liquid contact devices are the spikes in laboratory Vigreux fractionating columns. The trays or plates used in industrial distillation columns are fabricated of circular steel plates and usually installed inside the column at intervals of about 60 to 75 cm (24 to 30 inches) up the height of the column.
The introduction of paper chromatography was an important analytical technique which gave rise to thin-layer chromatography. [13] Finally, gas-liquid chromatography, a fundamental technique in modern analytical chemistry, was described by Martin with coauthors A. T. James and G. Howard Smith in 1952. [14]
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