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Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar , resulting in the formation of a visible precipitate when they interact.
Örjan Thomas Ouchterlony (January 14, 1914, Stockholm – September 25, 2004) was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test in the 1940s. [1] [2] He was trained at Karolinska Institute, where his received his medical doctorate.
Introductory video demonstrating radial immunodiffusion technique (3:43 minutes). "Radial Immunodiffusion (Mancini Technique)" (video). Frank Lectures. 2017-08-08. Archived from the original on 2021-12-21 – via YouTube. Introductory lecture/slideshow illustrating radial immunodiffusion theory and technique. (6:56 minutes)
Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens. [1] The specific region an antibody recognizes on an antigen is called an ...
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. It is a valuable tool for the determination of cellular contents from individual cells.
A schematic of a complement fixation test. The complement fixation test is an immunological medical test that can be used to detect the presence of either specific antibody or specific antigen in a patient's serum, based on whether complement fixation occurs.
Ouchterlony double immunodiffusion, an immunological technique named after Örjan Ouchterlony Topics referred to by the same term This disambiguation page lists articles associated with the title Ochterlony .