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Boric acid in equilibrium with its conjugate base the borate ion is widely used (in the concentration range 50–100 ppm boron equivalents) as a primary or adjunct pH buffer system in swimming pools. Boric acid is a weak acid, with pK a (the pH at which buffering is strongest because the free acid and borate ion are in equal concentrations) of ...
Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range. Boric acid has a pK a of 9.14 at 25 °C.
A mixture containing citric acid, monopotassium phosphate, boric acid, and diethyl barbituric acid can be made to cover the pH range 2.6 to 12. [ 8 ] Other universal buffers are the Carmody buffer [ 9 ] and the Britton–Robinson buffer , developed in 1931.
TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA.. In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis.
Universal buffers consist of mixtures of acids of diminishing strength (increasing pK a), so that the change in pH is approximately proportional to the amount of alkali added. It consists of a mixture of 0.04 M boric acid, 0.04 M phosphoric acid and 0.04 M acetic acid that has been titrated to the desired pH with 0.2 M sodium hydroxide. Britton ...
Borate ions (commonly supplied as boric acid) are used in biochemical and chemical laboratories to make buffers, e.g. for polyacrylamide gel electrophoresis of DNA and RNA, such as TBE buffer (borate buffered tris-hydroxymethylaminomethonium) [28] [29] or the newer SB buffer or BBS buffer (borate buffered
LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Lithium borate ( lithium hydroxide monohydrate and boric acid ).
Other formulations have been developed over time. In 1916, Marcel Daufresne substituted sodium bicarbonate for Dakin's boric acid as buffering agent. [7] [17] This formulation is the basis of current commercial products. [18] The concentration chosen by Dakin (0.5%) was the maximum highest concentration found tolerable to the skin.