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In 90% acetone-water, the peak absorption wavelengths of chlorophyll a are 430 nm and 664 nm; peaks for chlorophyll b are 460 nm and 647 nm; peaks for chlorophyll c 1 are 442 nm and 630 nm; peaks for chlorophyll c 2 are 444 nm and 630 nm; peaks for chlorophyll d are 401 nm, 455 nm and 696 nm.
Higher chlorophyll concentrations generally indicate areas of enhanced primary production, and conversely lower chlorophyll levels indicate low primary production. This co-occurrence of low chlorophyll and high macronutrient availability is why these regions are deemed "high-nutrient, low-chlorophyll."
The deep chlorophyll maximum (DCM), also called the subsurface chlorophyll maximum, [1] [2] is the region below the surface of water with the maximum concentration of chlorophyll. The DCM generally exists at the same depth as the nutricline, the region of the ocean where the greatest change in the nutrient concentration occurs with depth. [3]
Low-nutrient, low-chlorophyll (LNLC) regions are aquatic zones that are low in nutrients (such as nitrogen, phosphorus, or iron) and consequently have low rate of primary production, as indicated by low chlorophyll concentrations.
The Trophic State Index (TSI) is a classification system designed to rate water bodies based on the amount of biological productivity they sustain. [1] Although the term "trophic index" is commonly applied to lakes, any surface water body may be indexed. The TSI of a water body is rated on a scale from zero to one hundred. [1]
For example, definitions of blooms have included when the concentration of chlorophyll exceeds 100 ug/L, [6] when the concentration of chlorophyll exceeds 5 ug/L, [7] when the species considered to be blooming exceeds concentrations of 1000 cells/mL, [8] and when the algae species concentration simply deviates from its normal growth. [9] [10]
Chlorophyll in the water changes the way the water reflects and absorbs sunlight, allowing scientists to map the amount and location of phytoplankton. These measurements give scientists valuable insights into the health of the ocean environment, and help scientists study the ocean carbon cycle .
Chlorophyll α is the core photosynthetic pigment that all phytoplankton possess. Concentrations of this pigment, which can be measured remotely, is used as a proxy for phytoplankton biomass in a given location general, the more chlorophyll a, the more phytoplankton biomass, although the CHL a to C ratio May vary between species, and even within the species.