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The light excites an electron in the pigment P680 at the core of photosystem II, which is transferred to the primary electron acceptor, pheophytin, leaving behind P680 +. The energy of P680 + is used in two steps to split a water molecule into 2H + + 1/2 O 2 + 2e - ( photolysis or light-splitting ).
This chain of electron acceptors is known as an electron transport chain. When this chain reaches PSI, an electron is again excited, creating a high redox-potential. The electron transport chain of photosynthesis is often put in a diagram called the Z-scheme, because the redox diagram from P680 to P700 resembles the letter Z. [3]
The overall equation for the light-dependent reactions under the conditions of non-cyclic electron flow in green plants is: [27] 2 H 2 O + 2 NADP + + 3 ADP + 3 P i + light → 2 NADPH + 2 H + + 3 ATP + O 2
to NADPH or are used in non-cyclic electron flow. [1] DCMU is a chemical often used in laboratory settings to inhibit photosynthesis. When present, DCMU inhibits electron flow from photosystem II to plastoquinone.
At the reaction center, the electrons on the special chlorophyll molecule will be excited and ultimately transferred away by electron carriers. (If the electrons were not transferred away after excitation to a high energy state, they would lose energy by fluorescence back to the ground state, which would not allow plants to drive photosynthesis.)
This membrane contains an enzyme called NAD(P)H dehydrogenase which transfers electrons in a linear chain to oxygen molecules. [1] This electron transport chain (ETC) within the chloroplast also interacts with those in the mitochondria where respiration takes place. [2] Photosynthesis is also a process that Chlororespiration interacts with. [2]
These protons then flow down their electrochemical potential gradient through an enzyme called ATP-synthase, creating ATP by the phosphorylation of ADP to ATP. The electrons from the initial light reaction reach Photosystem I, then are raised to a higher energy level by light energy and then received by an electron acceptor and reduce NADP + to ...
It is possible to introduce an artificial electron acceptor into the light reaction, such as a dye that changes color when it is reduced. These are known as Hill reagents. These dyes permitted the finding of electron transport chains during photosynthesis. Dichlorophenolindophenol (DCPIP), an example of these dyes, is widely used by experimenters.