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  2. Auxotrophy - Wikipedia

    en.wikipedia.org/wiki/Auxotrophy

    In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...

  3. URA3 - Wikipedia

    en.wikipedia.org/wiki/URA3

    URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).

  4. Candida albicans - Wikipedia

    en.wikipedia.org/wiki/Candida_albicans

    Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The URA3 marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of C. albicans. [119]

  5. Kanamycin A - Wikipedia

    en.wikipedia.org/wiki/Kanamycin_A

    Mammalian cells, yeast, and other eukaryotes acquire resistance to geneticin (= G418, an aminoglycoside antibiotic similar to kanamycin) when transformed with a kanMX marker. In yeast, the kanMX marker avoids the requirement of auxotrophic markers. In addition, the kanMX marker renders E. coli resistant to kanamycin.

  6. YEPD - Wikipedia

    en.wikipedia.org/wiki/YEPD

    The yeast extract will typically contain all the amino acids necessary for growth. By being a complete medium, YEPD cannot be used as a selection medium to test for auxotrophs. Instead, YEPD is used as a growth medium to grow yeast cultures. YEPD typically contains 1% yeast extract, 2% peptone, and 2% glucose in distilled water.

  7. Malassezia pachydermatis - Wikipedia

    en.wikipedia.org/wiki/Malassezia_pachydermatis

    Malassezia pachydermatis is a zoophilic yeast in the division Basidiomycota.It was first isolated in 1925 by Fred Weidman, and it was named pachydermatis (Greek for 'thick-skin') after the original sample taken from an Indian rhinoceros (Rhinocerosus unicornis) with severe exfoliative dermatitis. [2]

  8. Buffered charcoal yeast extract agar - Wikipedia

    en.wikipedia.org/wiki/Buffered_charcoal_yeast...

    Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis , [ 2 ] Francisella and Nocardia spp .

  9. Torula - Wikipedia

    en.wikipedia.org/wiki/Torula

    Torula, in its inactive form (usually labeled as torula yeast), is widely used as a flavoring in processed foods and pet foods. It is often grown on wood liquor, a byproduct of paper production , which is rich in wood sugars ( xylose ). [ 3 ]

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