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Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology.
Several molecular typing schemes have been proposed to determine the relatedness of pathogens such as pulsed-field gel electrophoresis , ribotyping, and PCR-based fingerprinting. But these DNA banding-based subtyping methods do not provide meaningful evolutionary analyses.
Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining: Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a ...
Additionally, the tester must identify and verify methods for inactivating and reactivating the gene being studied. [2] In 1996, Fredricks and Relman proposed seven molecular guidelines for establishing microbial disease causation: [3] "A nucleic acid sequence belonging to a putative pathogen should be present in most cases of an infectious ...
The method is named after the British biologist Edwin Southern, who first published it in 1975. [3] Other blotting methods (i.e., western blot , [ 4 ] northern blot , eastern blot , southwestern blot ) that employ similar principles, but using RNA or protein, have later been named for compass directions as a sort of pun from Southern's name.