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Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1] [2] [3] [4]
The Kovats index of n-alkanes is 100 times their carbon number, e.g. the Kovats index of n-butane is 400. The Kovats index is dimensionless, unlike retention time or retention volume. For isothermal gas chromatography, the Kovats index is given by the equation:
In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor R F is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [ 2 ]
Retention time – the characteristic time it takes for a particular analyte to pass through the system (from the column inlet to the detector) under set conditions. See also: Kovats' retention index; Sample – the matter analyzed in chromatography. It may consist of a single component or it may be a mixture of components.
From the variables in the figure above, the resolution, plate number, and plate height of the column plate model can be calculated using the equations: Resolution (R s): R s = 2(t RB – t RA)/(w B + w A), where: t RB = retention time of solute B t RA = retention time of solute A w B = Gaussian curve width of solute B w A = Gaussian curve width ...
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The relative response factor is the expected ratio of an analyte to an internal standard (or external standard) and is calculated by finding the response of a known amount of analyte and a constant amount of internal standard (a chemical added to the sample at a constant concentration, with a distinct retention time to the analyte).
The most significant disruptions to sleep took place when 400 mg was consumed within 4 hours of bedtime — increasing the time it took for participants to fall asleep, and reducing total sleep ...