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  2. Primer walking - Wikipedia

    en.wikipedia.org/wiki/Primer_walking

    The first is called chromosome (or primer) walking and starts with sequencing the first piece. The next (contiguous) piece of the sequence is then sequenced using a primer which is complementary to the end of the first sequence read and so on. This technique doesn't require much assembling, but you need a lot of primers and it is relatively ...

  3. Paired-end tag - Wikipedia

    en.wikipedia.org/wiki/Paired-end_tag

    Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3' ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites ...

  4. List of sequenced eukaryotic genomes - Wikipedia

    en.wikipedia.org/wiki/List_of_sequenced...

    Saccharomyces cerevisiae was the first eukaryotic organism to have its complete genome sequence determined.. This list of "sequenced" eukaryotic genomes contains all the eukaryotes known to have publicly available complete nuclear and organelle genome sequences that have been sequenced, assembled, annotated and published; draft genomes are not included, nor are organelle-only sequences.

  5. Cap analysis of gene expression - Wikipedia

    en.wikipedia.org/wiki/Cap_Analysis_of_Gene...

    The small fragments (historically 27 nucleotides long, but now limited only by sequencing technologies) from the very beginnings of mRNAs (5' ends of capped transcripts) are extracted, reverse-transcribed to cDNA, PCR amplified (if needed) and sequenced. CAGE was first published by Hayashizaki, Carninci and co-workers in 2003. [1]

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    Sydney Sweeney hit back after body shamers piled on one of her Instagram posts, which included videos and photos of her hitting the gym to prepare for her role as boxer Christy Martin.

  7. Should You Buy PayPal Stock While It's Below $87? - AOL

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    But for the full year, it expects its adjusted EPS to grow by the "high teens" as its free cash flow rises 30% to roughly $6 billion. It plans to return that cash to its investors via $6 billion ...

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  9. Scaffolding (bioinformatics) - Wikipedia

    en.wikipedia.org/wiki/Scaffolding_(bioinformatics)

    Most high-throughput, next generation sequencing platforms produce shorter read lengths compared to Sanger sequencing.These new platforms are able to generate large quantities of data in short periods of time, but until methods were developed for de novo assembly of large genomes from short read sequences, Sanger sequencing remained the standard method of creating a reference genome. [10]

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