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Colocalization is used in real-time single-molecule fluorescence microscopy to detect interactions between fluorescently labeled molecular species. In this case, one species (e.g. a DNA molecule) is typically immobilized on the imaging surface, and the other species (e.g. a DNA-binding protein) is supplied to the solution.
The Colocalization Benchmark Source (CBS) is a free collection of downloadable images to test and validate the degree of colocalization of markers in any fluorescence microscopy studies. Colocalization is a visual phenomenon when two molecules of interest are associated with the same structures in the cells and potentially share common ...
This list of protein subcellular localisation prediction tools includes software, databases, and web services that are used for protein subcellular localization prediction.
IF colocalization studies Immunoblotting after subcellular fractionation PS exposure An early event of apoptosis is the PS exposure on the outer leaflet of the plasma membrane, but it may be reversible. PS exposure occurs also in T-cell activation, without cell death. FACS quantification of Annexin V binding
A fluorophore-labeled human cell. A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. . Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bon
Vaa3D was created in 2007 to tackle the large-scale brain mapping project at Janelia Farm of the Howard Hughes Medical Institute.The initial goal was to quickly visualize any of the tens of thousands of large 3D laser scanning microscopy image stacks of fruit fly brains, each with a few gigabytes in volume.
FHAD1 was found to interact (colocalization) with 14-3-3 protein epsilon via cosedimentation. This protein binds to a number of binding partners, mostly by recognizing phosphothreonine or phosphoserine motifs.
The image above depicts the conversion from a cosegregation matrix to an adjacency matrix is one use of a matrix in genome architecture mapping where scientists are using cryosectioning to find colocalization between DNA regions, genomes, and/or alleles. In that example, cosegregation is being used to describe the linkage of data to each other ...