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Integrated DNA Technologies, Inc. (IDT), headquartered in Coralville, Iowa, is a supplier of custom nucleic acids, serving the areas of academic research, biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacturing of custom DNA and RNA oligonucleotides (oligos) for research applications.
The result is a stem-loop primer that excludes annealing involving shorter overlaps, but permits annealing of the primer to its fully complementary sequence in the target. Chimeric primers: some DNA bases in the primer are replaced with RNA bases, creating a chimeric sequence. The melting temperature of a chimeric sequence with another chimeric ...
Buffer P2 is a lysis buffer solution produced by Qiagen.It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and 200mM NaOH.It is used in conjunction with other resuspension buffers and lysis buffers to release DNA from cells, often as part of the alkaline lysis method of purifying plasmid DNA from bacterial cell culture.
Upstream Color is a 2013 American experimental science fiction film written, directed, produced by and starring Shane Carruth.The film is the second feature directed by Carruth, following his 2004 debut Primer. [3]
Shane Carruth (born 1972) is an American filmmaker, screenwriter, composer, and actor. [1] [2] He is the writer, director, and co-star of the prize-winning science-fiction film Primer (2004), which was his debut feature.
Primer walking is a method to determine the sequence of DNA up to the 1.3–7.0 kb range whereas chromosome walking is used to produce the clones of already known sequences of the gene. [2] Too long fragments cannot be sequenced in a single sequence read using the chain termination method .
Primer (gasoline engine), a device on some petrol engines used to prime the engine with gasoline before starting it; Primer (molecular biology), a nucleic acid strand (or related molecule) that serves as a starting point for replication; Money-creation primer; Trap primer, a plumbing device or valve that adds water to traps
The Stochastic Neural Analog Reinforcement Calculator (SNARC) is a neural-net machine designed by Marvin Lee Minsky. [ 1 ] [ 2 ] Prompted by a letter from Minsky, George Armitage Miller gathered the funding (a few thousand dollars) for the project from the Office of Naval Research in the summer of 1951 with the work to be carried out by Minsky ...