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A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.
The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments and data, as devised by Bustin et al. in 2009. [1]
Reference ranges (reference intervals) for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples. Reference ranges for blood tests are studied within the field of clinical chemistry (also known as "clinical biochemistry", "chemical pathology" or "pure blood chemistry"), the ...
Quantitative PCR (qPCR) is used to measure the specific amount of target DNA (or RNA) in a sample. By measuring amplification only within the phase of true exponential increase, the amount of measured product more accurately reflects the initial amount of target.
In this article, RT-PCR will denote Reverse Transcription PCR. Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings. The close association between RT-PCR and qPCR has led to metonymic use of the term qPCR to mean RT-PCR.
Price on eBay: $8,500 Porcelain dolls don’t have to be more than 2 feet tall to be worth a lot of money. This little lady stands only 15 1/2 inches tall, but her ornate details and impressive ...
Taylor Kitsch is honoring his sister Shelby Kitsch-Best in a special way.. In the mid-2010s, the Friday Night Lights alum, 43, took two years off of work to help Kitsch-Best through her addiction ...
The third-quarter pass secured the first Titans touchdown of the day. The Vikings blitzed five pass rushers on third-and-10. But Tennessee's offensive line stood its ground to buy Levis time as he ...
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