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For example, pBR322 is a medium copy number plasmid (~20 copies/cell) from which several high copy number cloning vectors (>100 copies/cell) have been derived by mutagenesis, such as the well known pUC series. [1] This delivers the convenience of high plasmid DNA yields but the additional burden of the high copy number restricts the plasmid size.
Copy number analysis is the process of analyzing data produced by a test for DNA copy number variation in an organism's sample. One application of such analysis is the detection of chromosomal copy number variation that may cause or may increase risks of various critical disorders.
Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA. It is used in a wide array of biological experiments and practical applications ranging from genetic fingerprinting to large scale protein production.
P1 vectors also contain a P1 plasmid replicon, which ensures only one copy of the vector is present in a cell. However, there is a second P1 replicon- called the P1 lytic replicon- that is controlled by an inducible promoter. This promoter allows the amplification of more than one copy of the vector per cell prior to DNA extraction. [2] bac vector
Scientists have speculated about the Y-chromosomal haplogroup (and therefore patrilineal ancestry) of Genghis Khan.. Zerjal et al. (2003) identified a Y-chromosomal lineage haplogroup C*(xC3c) present in about 8% of men in a region of Asia "stretching from northeast China to Uzbekistan", which would be around 16 million men at the time of publication, "if [Zerjal et al's] sample is ...
This gene duplication has created a copy number variation. The chromosome now has two copies of this section of DNA, rather than one. Copy number variation (CNV) is a phenomenon in which sections of the genome are repeated and the number of repeats in the genome varies between individuals. [1]
DNA exists as a double-stranded structure, with both strands coiled together to form the characteristic double helix. Each single strand of DNA is a chain of four types of nucleotides. Nucleotides in DNA contain a deoxyribose sugar, a phosphate, and a nucleobase.
Low Copy Number (LCN) is a DNA profiling technique developed by the UK Forensic Science Service (FSS) which has been in use since 1999. [1]In the United Kingdom use of the technique was suspended between 21 December 2007 and 14 January 2008 while the Crown Prosecution Service conducted a review into its use – this suspension has now been lifted.