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Deoxyribonuclease II (EC 3.1.22.1, DNase II, pancreatic DNase II, deoxyribonucleate 3'-nucleotidohydrolase, pancreatic DNase II, acid deoxyribonuclease, acid DNase) is an endonuclease that hydrolyzes phosphodiester linkages of deoxyribonucleotide in native and denatured DNA, yielding products with 3'-phosphates and 5'-hydroxyl ends, which occurs as a result of single-strand cleaving mechanism. [1]
DNase I contains four ion-binding pockets, and requires Ca 2+ and Mg 2+ for hydrolyzing double-stranded DNA. [5] Two of the sites strongly bind Ca 2+ while the other two coordinate Mg 2+ . Little has been published on the number and location of the Mg 2+ binding sites, although it has been proposed that Mg 2+ is located near the catalytic ...
Gene conversion is the process by which one DNA sequence replaces a homologous sequence such that the sequences become identical after the conversion. [1] Gene conversion can be either allelic, meaning that one allele of the same gene replaces another allele, or ectopic, meaning that one paralogous DNA sequence converts another.
Ribonuclease (commonly abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7 (for the phosphorolytic enzymes) and 3.1 (for the hydrolytic enzymes) classes of enzymes.
In addition, as noted by Wilkins and Holliday, [9] there are four novel steps needed in meiosis that are not present in mitosis. These are: (1) pairing of homologous chromosomes , (2) extensive recombination between homologs; (3) suppression of sister chromatid separation in the first meiotic division; and (4) avoiding chromosome replication ...
Intentional breaks are generated as intermediaries in meiosis and V(D)J recombination, which are primarily repaired through homologous recombination and non-homologous end joining. Both cases require the ends in double strand breaks be processed by nucleases before repair can take place. One such nuclease is Mre11 complexed with Rad50.
In molecular biology, endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain (namely DNA or RNA).Some, such as deoxyribonuclease I, cut DNA relatively nonspecifically (with regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences.
During the phase of meiosis labeled “interphase s” in the meiosis diagram there is a round of DNA replication, so that each of the chromosomes initially present is now composed of two copies called chromatids. These chromosomes (paired chromatids) then pair with the homologous chromosome (also paired chromatids) present in the same nucleus ...