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  2. Bayesian tool for methylation analysis - Wikipedia

    en.wikipedia.org/wiki/Bayesian_tool_for...

    Bayesian tool for methylation analysis, also known as BATMAN, is a statistical tool for analysing methylated DNA immunoprecipitation (MeDIP) profiles. It can be applied to large datasets generated using either oligonucleotide arrays (MeDIP-chip) or next-generation sequencing (MeDIP-seq), providing a quantitative estimation of absolute methylation state in a region of interest.

  3. Combined bisulfite restriction analysis - Wikipedia

    en.wikipedia.org/wiki/Combined_Bisulfite...

    The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites. Combined Bisulfite Restriction Analysis (or COBRA) is a molecular biology technique that allows for the sensitive quantification of DNA methylation levels at a specific genomic locus on a DNA sequence in a small sample of genomic DNA. [1]

  4. Bisulfite sequencing - Wikipedia

    en.wikipedia.org/wiki/Bisulfite_sequencing

    Bisulfite sequencing applies routine sequencing methods on bisulfite-treated genomic DNA to determine methylation status at CpG dinucleotides. Other non-sequencing strategies are also employed to interrogate the methylation at specific loci or at a genome-wide level. All strategies assume that bisulfite-induced conversion of unmethylated ...

  5. Whole genome bisulfite sequencing - Wikipedia

    en.wikipedia.org/wiki/Whole_genome_bisulfite...

    The DNA methylation status at various genes can reveal information regarding gene regulation and transcriptional activities. [1] This technique was developed in 2009 along with reduced representation bisulfite sequencing after bisulfite sequencing became the gold standard for DNA methylation analysis. [2] [3]

  6. Reduced representation bisulfite sequencing - Wikipedia

    en.wikipedia.org/wiki/Reduced_representation_bi...

    Enzyme digestion: First, genomic DNA is digested using a methylation-insensitive restriction enzyme. It is integral for the enzymes to not be influenced by the methylation status of the CpGs (sites within the genome where a cytosine is next to a guanine) as this allows for the digestion of both methylated and unmethylated areas.

  7. Differentially methylated region - Wikipedia

    en.wikipedia.org/wiki/Differentially_methylated...

    Differentially methylated regions (DMRs) are genomic regions with different DNA methylation status across different biological samples and regarded as possible functional regions involved in gene transcriptional regulation. The biological samples can be different cells/tissues within the same individual, the same cell/tissue at different times ...

  8. NOMe-seq - Wikipedia

    en.wikipedia.org/wiki/NOMe-seq

    NOMe-seq has the additional benefit of providing DNA methylation status, which also plays a crucial role in the regulation of genomic activity. Interestingly, increased DNA methylation is associated with transcriptional silencing whereas accessible DNA unbound by nucleosomes is generally associated with transcriptional activation.

  9. Illumina Methylation Assay - Wikipedia

    en.wikipedia.org/wiki/Illumina_Methylation_Assay

    The Illumina Methylation Assay using the Infinium I platform uses 'BeadChip' technology [clarification needed] to generate a comprehensive genome-wide profiling of human DNA methylation. Similar to bisulfite sequencing and pyrosequencing , this method quantifies methylation levels at various loci within the genome .