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The CFU/plate is read from a plate in the linear range, and then the CFU/g (or CFU/mL) of the original is deduced mathematically, factoring in the amount plated and its dilution factor. A solution of bacteria at an unknown concentration is often serially diluted in order to obtain at least one plate with a countable number of bacteria.
Cell spreaders. In microbiology, a cell spreader or plate spreader is a tool used to smoothly spread cells and bacteria on a culture plate, such as a petri dish.. Cell spreaders can be made from glass, plastic, or metal, and come in various shapes.
Argo Bromo Anggrek is the name of executive and luxury train jointly operated by Kereta Api Indonesia between Surabaya and Jakarta in Indonesia. The train covers 720 kilometres (447 mi) in 8 hours and 5 minutes along Java 's north coast, stopping at Bojonegoro , Semarang Tawang , Pekalongan , and Cirebon . [ 1 ]
In each sector, 1 x 20 μl of the appropriate dilution is dropped onto the surface of the agar and the drop allowed to spread naturally. In the original description of the method a drop from a height of 2.5 cm spread over an area of 1.5-2.0 cm. It is important to avoid touching the surface of the agar with the pipette.
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria.
YPD medium bottle and YPD agar plate. YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1]
Intraplate deformation is the folding, breaking, or flow of the Earth's crust within plates instead of at their margins. This process usually occurs in areas with especially weak crust and upper mantle, such as the Tibetan Plateau (Figure 1). Intraplate deformation brings another aspect to plate tectonic theory.