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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.
Cell spreaders. In microbiology, a cell spreader or plate spreader is a tool used to smoothly spread cells and bacteria on a culture plate, such as a petri dish.. Cell spreaders can be made from glass, plastic, or metal, and come in various shapes.
Bacterial lawn is a term used by microbiologists to describe the appearance of bacterial colonies when all the individual colonies on a Petri dish or agar plate merge to form a field or mat of bacteria. Bacterial lawns find use in screens for antibiotic resistance and bacteriophage titering.
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
In each sector, 1 x 20 μl of the appropriate dilution is dropped onto the surface of the agar and the drop allowed to spread naturally. In the original description of the method a drop from a height of 2.5 cm spread over an area of 1.5-2.0 cm. It is important to avoid touching the surface of the agar with the pipette.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria.