Search results
Results from the WOW.Com Content Network
Denaturation: If alkaline transfer methods are used, the DNA gel is placed into an alkaline solution (typically containing sodium hydroxide) to denature the double-stranded DNA. The denaturation in an alkaline environment may improve binding of the negatively charged thymine residues of DNA to a positively charged amino groups of membrane ...
Single-stranded DNA or RNA tends to fold up into molecules with complex shapes and migrate through the gel in a complicated manner based on their tertiary structure. Therefore, agents that disrupt the hydrogen bonds, such as sodium hydroxide or formamide, are used to denature the nucleic acids and cause them to behave as long rods again. [26]
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
A chaotropic agent is a molecule in water solution that can disrupt the hydrogen bonding network between water molecules (i.e. exerts chaotropic activity).This has an effect on the stability of the native state of other molecules in the solution, mainly macromolecules (proteins, nucleic acids) by weakening the hydrophobic effect.
Sodium dodecyl sulfate detergent (SDS) and Sodium Hydroxide are used to lyse the cells. Sodium Hydroxide establishes an alkaline environment that disrupts the phospholipid bilayer and breaks hydrogen bonds between double-stranded chromosomal DNA, making it single-stranded. Due to the supercoiled nature of plasmid DNA, strands of the circular ...
The highest DNA adsorption efficiencies occur in the presence of buffer solution with a pH at or below the pKa of the surface silanol groups. The mechanism behind DNA adsorption onto silica is not fully understood; one possible explanation involves reduction of the silica surface's negative charge due to the high ionic strength of the buffer.
To precipitate the DNA out of the water, the negatively charged phosphate groups of the DNA backbone are neutralized by the addition of positively charged ions from a salt. But because of the high polarity of water, illustrated by its high dielectric constant of 80.1 (at 20 °C), the positively charged ions are shielded and unable to interact ...
The collection of DNA molecules of various truncated lengths therefore informs the frequency of reaction at every base position, which reflects the structure profile along the RNA. This is traditionally assayed by running the DNA on a gel , and the intensity of bands inform the frequency of observing a truncation at each position.