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Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar , resulting in the formation of a visible precipitate when they interact.
Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. The agarose was ...
Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.
Radial immunodiffusion (RID), Mancini immunodiffusion or single radial immunodiffusion assay, is an older immunodiffusion technique used in immunology to determine the quantity or concentration of an antigen in a sample.
Basic concept of Primary Immunofluorescence: An antibody with a conjugated fluorophore, that is specifically bound to an epitope on the target molecule.. Primary (direct) immunofluorescence (DIF) uses a single antibody, conjugated to a fluorophore.
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Antigen-antibody interaction, or antigen-antibody reaction, is a specific chemical interaction between antibodies produced by B cells of the white blood cells and antigens during immune reaction.
The following outline is provided as an overview of and topical guide to immunology: . Immunology – study of all aspects of the immune system in all organisms. [1] It deals with the physiological functioning of the immune system in states of both health and disease; malfunctions of the immune system in immunological disorders (autoimmune diseases, hypersensitivities, immune deficiency ...