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  2. 3' mRNA-seq - Wikipedia

    en.wikipedia.org/wiki/3'_mRNA-seq

    3' mRNA-seq is a quantitative, genome-wide transcriptomic technique based on the barcoding of the 3' untranslated region (UTR) of mRNA molecules. Unlike standard bulk RNA-seq, where short sequencing reads are generated along the entire length of mRNA transcripts, only the 3' end of polyadenylated RNAs are sequenced in 3' mRNA-seq.

  3. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    RNA-Seq can also be used to determine exon/intron boundaries and verify or amend previously annotated 5' and 3' gene boundaries. Recent advances in RNA-Seq include single cell sequencing, bulk RNA sequencing, [6] 3' mRNA-sequencing, in situ sequencing of fixed tissue, and native RNA molecule sequencing with single-molecule real-time sequencing. [7]

  4. Transcriptome - Wikipedia

    en.wikipedia.org/wiki/Transcriptome

    RNA sequencing is a next-generation sequencing technology; as such it requires only a small amount of RNA and no previous knowledge of the genome. [3] It allows for both qualitative and quantitative analysis of RNA transcripts, the former allowing discovery of new transcripts and the latter a measure of relative quantities for transcripts in a ...

  5. Three prime untranslated region - Wikipedia

    en.wikipedia.org/wiki/Three_prime_untranslated...

    (See Central dogma of molecular biology). mRNA structure, approximately to scale for a human mRNA, where the median length of 3′UTR is 700 nucleotides. In molecular genetics, the three prime untranslated region (3′-UTR) is the section of messenger RNA (mRNA) that immediately follows the translation termination codon.

  6. BRB-seq - Wikipedia

    en.wikipedia.org/wiki/BRB-seq

    The fundamental aspect of BRB-seq is the optimized sample barcode primers. Each barcoded nucleotide sequence includes an adaptor for primer annealing, a 14-nt long barcode that assigns a unique identifier to each individual RNA sample, and a random 14-nt long UMI that tags each mRNA molecule with a unique sequence to distinguish between original mRNA transcripts and duplicates that result from ...

  7. NYT ‘Connections’ Hints and Answers Today, Wednesday, December 11

    www.aol.com/nyt-connections-hints-answers-today...

    today's connections game answers for wednesday, december 11, 2024: 1. utopia: paradise, seventh heaven, shangri-la, xanadu 2. things you shake: hairspray, magic 8 ...

  8. Transcriptomics technologies - Wikipedia

    en.wikipedia.org/wiki/Transcriptomics_technologies

    Sequencing technology platforms commonly used for RNA-Seq [77] [78] Platform Commercial release Typical read length Maximum throughput per run Single read accuracy RNA-Seq runs deposited in the NCBI SRA (Oct 2016) [79] 454 Life Sciences: 2005 700 bp 0.7 Gbp 99.9% 3548 Illumina: 2006 50–300 bp 900 Gbp 99.9% 362903 SOLiD: 2008 50 bp 320 Gbp 99. ...

  9. When does 'No Good Deed' come out? How to watch Ray ... - AOL

    www.aol.com/does-no-good-deed-come-200044366.html

    All eight half-hour episodes of "No Good Deed" will be available to stream on Netflix at 12 a.m. PT / 3 a.m. ET on Thursday, Dec. 12. 'No Good Deed' cast (L to R) Lisa Kudrow as Lydia, Abbi ...