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Glucose oxidase is widely used coupled to peroxidase reaction that visualizes colorimetrically the formed H 2 O 2, for the determination of free glucose in sera or blood plasma for diagnostics, using spectrometric assays manually or with automated procedures, and even point-of-use rapid assays.
The Trinder glucose activity test is a diagnostic test used in medicine to determine the presence of glucose or glucose oxidase. The test employs the Trinder reagent , and is a colour change test resulting from the Trinder reaction .
Glucose concentrations vary in an individual, and a healthy person can present with transitory glucosuria after a meal high in sugars; therefore the most representative results come from samples obtained at least two hours after food is eaten. The detection of glucose by test strips is based on the enzymatic reaction of glucose oxidase.
Test strips for glucose contain the enzyme glucose oxidase, which breaks down glucose and forms hydrogen peroxide as a byproduct. In the presence of a peroxidase enzyme, hydrogen peroxide reacts with a chromogen to induce a color change. [51] The presence of glucose in the urine is known as glycosuria.
American Society for Microbiology, Oxidase Test Protocol. 2013. ASM MicrobeLibrary, 1–9. Cheng W J, Lin C W, Wu T G, Su C S, Hsieh M S. 2013. Calibration of glucose oxidase-based test strips for capillary blood measurement with oxygen saturated venous blood samples. Clinica Chimica Acta. 415, 152–157.
Glucose homeostasis, when operating normally, restores the blood sugar level to a narrow range of about 4.4 to 6.1 mmol/L (79 to 110 mg/dL) (as measured by a fasting blood glucose test). [ 10 ] The global mean fasting plasma blood glucose level in humans is about 5.5 mmol/L (100 mg/dL); [ 11 ] [ 12 ] however, this level fluctuates throughout ...
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Enzymes used in ELISAs include horseradish peroxidase (HRP), alkaline phosphatase (AP) or glucose oxidase. These enzymes allow for detection often because they produce an observable color change in the presence of certain reagents. In some cases these enzymes are exposed to reagents which cause them to produce light or chemiluminescence.
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