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For example, milk sugar (lactose) is a disaccharide made by condensation of one molecule of each of the monosaccharides glucose and galactose, whereas the disaccharide sucrose in sugar cane and sugar beet, is a condensation product of glucose and fructose. Maltose, another common disaccharide, is condensed from two glucose molecules. [7]
The common disaccharides lactose and maltose are directly detected by Benedict's reagent because each contains a glucose with a free reducing aldehyde moiety after isomerization. Sucrose (table sugar ) contains two sugars (fructose and glucose) joined by their glycosidic bond in such a way as to prevent the glucose undergoing isomerization to ...
Lactase (breaks down lactose into glucose and galactose) Maltase (breaks down maltose into 2 glucoses) Sucrase (breaks down sucrose into glucose and fructose) Trehalase (breaks down trehalose into 2 glucoses) For a thorough scientific overview of small-intestinal disaccharidases, one can consult chapter 75 of OMMBID. [1]
Reducing disaccharides like lactose and maltose have only one of their two anomeric carbons involved in the glycosidic bond, while the other is free and can convert to an open-chain form with an aldehyde group. The aldehyde functional group allows the sugar to act as a reducing agent, for example, in the Tollens' test or Benedict's test.
Lactose, maltose, and sucrose are all compound sugars, disaccharides, with the general formula C 12 H 22 O 11. They are formed by the combination of two monosaccharide molecules with the exclusion of a molecule of water. [72] Lactose is the naturally occurring sugar found in milk. A molecule of lactose is formed by the combination of a molecule ...
Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu 2 O), which forms a brick-red precipitate. [1] [2] RCHO + 2Cu 2+ + 2H 2 O → RCOOH + Cu 2 O↓ + 4H + (Disaccharides may also react, but the reaction is much slower.)
Maltose (/ ˈ m ɔː l t oʊ s / [2] or / ˈ m ɔː l t oʊ z / [3]), also known as maltobiose or malt sugar, is a disaccharide formed from two units of glucose joined with an α(1→4) bond. In the isomer isomaltose , the two glucose molecules are joined with an α(1→6) bond.
Salkowski test can also be used to test the presence of indoles (crystalline alkaloids that are a degradation products of proteins, containing tryptophan [10]). In such cases a sample is treated with nitric acid and 2% solution of potassium nitrite , with positive reaction being shown by presence of red colour.
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