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This likely allows TET1 to demethylate an adjacent methylated cytosine. When human mammary epithelial cells (MCF-10A) were treated with H 2 O 2, 8-OHdG increased in DNA by 3.5-fold and this caused about 80% demethylation of the 5-methylcytosines in the MCF-10A genome. [25]
This likely allows TET1 to demethylate an adjacent methylated cytosine. When human mammary epithelial cells (MCF-10A) were treated with H 2 O 2, 8-OHdG increased in DNA by 3.5-fold and this caused large scale demethylation of 5-methylcytosine to about 20% of its initial level in DNA. [17]
Cell culture in a small Petri dish Epithelial cells in culture, stained for keratin (red) and DNA (green). Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment.
To figure out how S phase entry can be affected by sporadic pulses of ERK activity at low EGF concentrations, they used MCF-10A cells co-expressing EKAR-EV and RFP-geminin and identified the pulses of ERK activity with the scoring and then align this ERK activity profiles with time of GFP-geminin induction.
Chicken cystatin quickly passed the membrane of MCF-10A neo T cells and inhibited cathepsin B when it was acylated with fatty acyl residues of 6-18 carbon atoms. [7] ...
The forced expression of oncogenic Ras in cultured human MCF-10A breast cancer cells markedly up-regulates BLT2 receptors and this up-regulation appears essential for the epithelial–mesenchymal transition-promoting ability of transforming growth factor beta in these cells; BLT2 receptors in these cells appear to stimulate the production of ...
The decoction of aerial parts of F. leucopyrus shows cytotoxic effects on breast cancer cells, particularly Her2 negative cells (MCF-7 and MDA-MB-231), compared to Her2 positive (SKBR-3) and non-cancerous cells (MCF-10A). This supports its traditional use for its anticancer properties.