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  2. Elution - Wikipedia

    en.wikipedia.org/wiki/Elution

    Elution principle of column chromatography. In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from a gel electrophoresis or chromatography column.

  3. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Chromatography – a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. Eluent (sometimes spelled eluant) – the solvent or solvent fixure used in elution chromatography and is synonymous with mobile phase. [11]

  4. Size-exclusion chromatography - Wikipedia

    en.wikipedia.org/wiki/Size-exclusion_chromatography

    Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers . [ 3 ]

  5. Column chromatography - Wikipedia

    en.wikipedia.org/wiki/Column_chromatography

    Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.

  6. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    Displacement chromatography has advantages over elution chromatography in that components are resolved into consecutive zones of pure substances rather than "peaks". Because the process takes advantage of the nonlinearity of the isotherms, a larger column feed can be separated on a given column with the purified components recovered at ...

  7. Displacement chromatography - Wikipedia

    en.wikipedia.org/wiki/Displacement_chromatography

    In any form of chromatography, the rate at which the solute moves down the column is a direct reflection of the percentage of time the solute spends in the mobile phase. To achieve separation in either elution or displacement chromatography, there must be appreciable differences in the affinity of the respective solutes for the stationary phase.

  8. Ion chromatography - Wikipedia

    en.wikipedia.org/wiki/Ion_chromatography

    Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [ 2 ] large proteins , [ 3 ] small nucleotides , [ 4 ] and amino acids .

  9. Anion-exchange chromatography - Wikipedia

    en.wikipedia.org/wiki/Anion-exchange_chromatography

    Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). [2] In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules ...

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