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Interior of the United States Department of Defense Serum Repository (DoDSR).. The Department of Defense Serum Repository (also referred to as the DoD Serum Repository or simply DoDSR) is a biological repository operated by the United States Department of Defense containing over 50,000,000 human serum specimens, collected primarily from applicants to and members of the United States Uniformed ...
Human platelet lysate (or hPL) is a substitute supplement for fetal bovine serum (FBS) in experimental and clinical cell culture. It is a turbid, light-yellow liquid that is obtained from human blood platelets after freeze/thaw cycle(s).
Cryoprecipitate, also called cryo for short, or Cryoprecipitate Antihemophilic factor (AHF), is a frozen blood product prepared from blood plasma. [1] To create cryoprecipitate, Plasma is slowly thawed to 1–6 °C. A cold-insoluble precipitate is formed, which is collected by centrifugation, resuspended in a small amount of residual plasma ...
Serum sample preparation requires about 30 minutes of waiting time before it can be centrifuged and then analyzed. [23] However, coagulation can be hastened down to a few minutes by adding thrombin or similar agents to the serum sample. [25] Compared to serum, 15–20% larger volume of plasma can be obtained from a blood sample of certain size.
The first human corpse to be frozen with the hope of future resurrection was James Bedford's, a few hours after his cancer-caused death in 1967. [15] Bedford's is the only cryonics corpse frozen before 1974 still frozen today.
In plasma or serum (g/cm 3) Water: Solvent 0.81-0.86 0.93-0.95 Acetoacetate: Produced in liver 8-40 × 10 −7: 4-43 × 10 −7: Acetone: product of bodyfat breakdown 3-20 × 10 −6: Acetylcholine: Neurotransmitter of the parasympathetic nervous system: 6.6-8.2 × 10 −8: Adenosine triphosphate: Energy storage total 3.1-5.7 × 10 −4 ...
The Cohn process, developed by Edwin J. Cohn, is a series of purification steps with the purpose of extracting albumin from blood plasma.The process is based on the differential solubility of albumin and other plasma proteins based on pH, ethanol concentration, temperature, ionic strength, and protein concentration.
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.