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The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo.It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 μ plasmid of baker's yeast ...
It is an early example of an operating system using basic object-oriented principles and garbage collection on the system level and a document centered approach for the user interface (UI), as envisaged later with OpenDoc. Ceres was a follow-up project to the Lilith workstation, based on AMD bit slicing technology and the programming language ...
In yeast, deletion strains are frequently used to assess protein stability over time with cycloheximide chases. For example, yeast strains lacking critical degradation machinery such as chaperones, E3 ligases, and vacuolar proteins are often used to determine the mechanism of degradation for a protein substrate of interest.
Nobel prize winner Eduard Buchner was arguably the first to present a cell-free system using yeast extracts, but since then alternative sources have been found. [9] [10] E. coli, wheat germ, and rabbit reticulocytes have all proven useful to create cell-free systems by extraction of their interior components.
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FBA formalizes the system of equations describing the concentration changes in a metabolic network as the dot product of a matrix of the stoichiometric coefficients (the stoichiometric matrix S) and the vector v of the unsolved fluxes. The right-hand side of the dot product is a vector of zeros representing the system at steady state. At steady ...