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Cell lysis is used in laboratories to break open cells and purify or further study their contents. Lysis in the laboratory may be affected by enzymes or detergents or other chaotropic agents . Mechanical disruption of cell membranes, as by repeated freezing and thawing, sonication , pressure, or filtration may also be referred to as lysis.
Sonoporation, or cellular sonication, is the use of sound in the ultrasonic range for increasing the permeability of the cell plasma membrane. This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or ...
Sonication can be used for the production of nanoparticles, such as nanoemulsions, [5] nanocrystals, liposomes and wax emulsions, as well as for wastewater purification, degassing, extraction of seaweed polysaccharides [1] and plant oil, extraction of anthocyanins and antioxidants, [6] production of biofuels, crude oil desulphurization, cell disruption, polymer and epoxy processing, adhesive ...
The French pressure cell press, or French press, is an apparatus used in biological experimentation to disrupt the plasma membrane of cells by passing them through a narrow valve under high pressure. [1] The French press can also be used for disintegration of chloroplasts, homogenates of animal tissue, and other biological particles.
Depending on how fragile the protein is and how stable the cells are, one could, for instance, use one of the following methods: i) repeated freezing and thawing, ii) sonication, iii) homogenization by high pressure (French press), iv) homogenization by grinding (bead mill), and v) permeabilization by detergents (e.g. Triton X-100) and/or ...
It has since been used in other applications such as cell disruption nanoemulsions, and solid particle size reduction, among others. By using microchannels with fixed geometry, and an intensifier pump, high shear rates are generated that rupture the cells. This method of cell lysis can yield breakage of over 90% of E. coli cells. [9]
A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction).
Sonication is often employed to provide local mixing of streams through the generation of ultra-high energy acoustics. [26] Microfluidic chips utilizing sonication mixing can have both integrated and externally located ultrasonic transducers. [27] Sonication is also used widely for cell lysis and homogenization in both macro and microfluidic ...
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