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A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. [1][2] Many methods have been developed to purify plasmid DNA from bacteria. [1][3] During the purification ...
This partitioning process is similar to that which occurs during a liquid–liquid extraction but is continuous, not step-wise. [ citation needed ] In the example using a water/acetonitrile gradient, the more hydrophobic components will elute (come off the column) later, then, once the mobile phase gets richer in acetonitrile ( i.e. , in a ...
Liquid–liquid extraction, also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar). There is a net transfer of one or more species from one liquid into another ...
A 12% solution is widely used in waterworks for the chlorination of water, and a 15% solution is more commonly [39] used for disinfection of waste water in treatment plants. Sodium hypochlorite can also be used for point-of-use disinfection of drinking water, [ 40 ] taking 0.2–2 mg of sodium hypochlorite per liter of water.
A form of budding called suckering is the reproduction or regeneration of a plant by shoots that arise from an existing root system. Species that characteristically produce suckers include elm ( Ulmus ) [ 4 ] : 299 and many members of the rose family such as Rosa , [ 4 ] : 285–296 Kerria [ 4 ] : 206 and Rubus .
Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA, and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding salt and ethanol as an antisolvent. In DNA extraction, after separating DNA from other cell constituents in water, DNA is precipitated out of solution by neutralizing it with positively ...
McCabe–Thiele method. The McCabe–Thiele method is a technique that is commonly employed in the field of chemical engineering to model the separation of two substances by a distillation column. [1][2][3] It uses the fact that the composition at each theoretical tray is completely determined by the mole fraction of one of the two components.
The sample solution is then distilled with a small amount of sodium hydroxide (NaOH). [3] NaOH can also be added with a dropping funnel. [4] NaOH reacts the ammonium (NH 4 +) to ammonia (NH 3), which boils off the sample solution. Ammonia bubbles through the standard acid solution and reacts back to ammonium salts with the weak or strong acid. [3]