Search results
Results from the WOW.Com Content Network
A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λ max = 443 nm [2]) to red (λ max = 570 nm [3]) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink color. [4] [5]
A pH indicator is a halochromic chemical compound added in small amounts to a solution so the pH (acidity or basicity) of the solution can be determined visually or spectroscopically by changes in absorption and/or emission properties. [1] Hence, a pH indicator is a chemical detector for hydronium ions (H 3 O +) or hydrogen ions (H +) in the ...
The doubly deprotonated (In 2-) phenolate form (the anion form of phenol) gives the familiar pink color. In strongly basic solutions, phenolphthalein is converted to its In(OH) 3− form, and its pink color undergoes a rather slow fading reaction [6] and becomes completely colorless when pH is greater than 13.
Solution: The main components of a universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue, and phenolphthalein. This mixture is important because each component loses or gains protons depending upon the acidity or alkalinity of the solution being tested. It is beneficial to use this type of universal ...
Chlorophenol red is an indicator dye that changes color from yellow to violet in the pH range 5.4 to 6.8. [2] The pH of a substance is determined by taking the negative logarithm of the Hydronium ion concentration and the indictor changes color due to the dissociation of H + ions. [3] The lambda max is at 572 nm. [4]
Phenol is a component in liquid–liquid phenol–chloroform extraction technique used in molecular biology for obtaining nucleic acids from tissues or cell culture samples. Depending on the pH of the solution either DNA or RNA can be extracted. Phenol is so inexpensive that it also attracts many small-scale uses.
This category contains articles about pH indicators: chemical compounds which change colour or fluorescence in response to changes in pH Wikimedia Commons has media related to PH indicators . Subcategories
Many different formulations exist. Typically, one liter of RPMI 1640 contains: [2] Glucose (2 g) A pH indicator (phenol red, 5 mg)Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)